Anti-TRAF6 Antibody

This polyclonal antibody from Antibodies.com targets TRAF6 (TNF Receptor-Associated Factor 6), an E3 ubiquitin ligase and adaptor protein that mediates signaling from the TNF receptor superfamily, Toll-like receptors, and the IL-1 receptor family. TRAF6 catalyzes K63-linked polyubiquitination to drive NF-κB and MAPK activation, positioning it as a central node in innate immunity, inflammatory responses, and osteoclast differentiation.

Raised in rabbit and supplied as 100 µl of unconjugated polyclonal antiserum, the reagent supports western blot, immunohistochemistry, and ELISA workflows. Validation has been confirmed for human, mouse, and rat samples, enabling cross-species studies of inflammatory signaling, bone resorption research, and downstream analysis of immune receptor pathways.

Typical applications include probing TRAF6 levels in stimulated macrophage or osteoclast lysates by western blot, localizing the protein in tissue sections by immunohistochemistry, and running quantitative ELISA assays measuring induced TRAF6 expression in cell culture models. The unconjugated format permits flexible pairing with secondary antibodies for fluorescence or chromogenic detection. For handling, refrigerate the vial upon receipt, aliquot to limit freeze-thaw cycles, and protect from light when not in use.