Anti-TRADD Antibody

The TRADD protein (TNFRSF1A-associated death domain protein) functions as a critical adapter in tumor necrosis factor receptor 1 signaling, where it bridges TNFR1 activation to two principal downstream outcomes: NF-κB-driven transcription and caspase-mediated apoptosis. This unconjugated rabbit polyclonal reagent from Antibodies.com is raised against the human target and supplied as a 100 µl liquid aliquot, supporting western blot and immunohistochemistry workflows on cell lysates and tissue preparations.

The antibody detects endogenous human TRADD and is commonly deployed in studies of TNF-driven inflammation, receptor-proximal signalosome assembly, and programmed cell death pathways. Polyclonal rabbit immunoglobulins recognize multiple epitopes across TRADD, an advantage when probing alternative isoforms or post-translationally modified forms. The unconjugated format leaves the choice of reporter open: HRP, biotin, fluorophore, or other secondary chemistries can be paired with the primary reagent as the experimental design demands.

For western blot, working dilutions are established empirically against positive-control lysates from cell lines known to express TRADD. For IHC, antigen retrieval and chromogen conditions should be validated on TRADD-positive and TRADD-negative control tissues before experimental samples are processed.