Anti-TP53 Antibody [Assigned #A10610]

The TP53 tumor suppressor is one of the most studied proteins in cancer biology, and monoclonal antibodies against it are commonly used to map its expression, mutation status, and signaling context across research workflows. This unconjugated mouse IgG clone is raised against human TP53 and is validated for three routine detection platforms: Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF). Investigators studying DNA damage response, apoptosis pathways, or p53 stabilization in cell culture and tissue specimens rely on consistent p53 detection, and a monoclonal reagent reduces the lot-to-lot variability that polyclonal sera can introduce when tracking subcellular localization or post-translational modification patterns.

Each vial from Antibodies.com is supplied as 200 µl of unconjugated antibody, an amount sized for typical immunoblotting and staining workflows. The mouse host and IgG isotype give access to a broad secondary antibody menu, letting users pair the primary with enzyme- or fluorophore-conjugated secondaries matched to their chosen detection chemistry. Because p53 expression and stability shift rapidly under cellular stress, aliquoting upon first thaw and storage at refrigerated temperatures helps preserve binding activity across repeated experiments, and limiting freeze-thaw cycles supports epitope recognition over time.

Reactivity is confirmed against human TP53, supporting work with cancer cell line panels, patient-derived xenograft material, and tumor microarray sections where p53 status is being characterized. The unconjugated format also allows the antibody to be used in custom labeling workflows or in co-localization studies alongside directly conjugated primaries.