Anti-TP53 Antibody [Assigned #A10610]

Produced by Antibodies.com as a Mouse IgG monoclonal, this unconjugated primary antibody binds human TP53, the 53 kDa tumor-suppressor protein widely studied in cancer biology and DNA damage response research. Each vial contains 100 µl of liquid reagent, sufficient for multiple western blot, immunohistochemistry, and immunofluorescence experiments on cultured cells or tissue sections.

The TP53 target (also known as p53) acts as a transcription factor that arrests the cell cycle, triggers apoptosis, and drives senescence in response to genotoxic stress, which is why immunodetection of p53 is a staple of oncology and toxicology studies. With monoclonal specificity and a mouse IgG isotype, the reagent delivers consistent lot-to-lot performance and pairs readily with HRP-, fluorophore-, or biotin-conjugated secondary antibodies of the user's choice, supporting chromogenic DAB staining, fluorescence imaging, and chemiluminescent western blot detection in turn.

Validated for use on human samples, the antibody is applied to standard cell-line models of p53 biology such as U2OS, MCF7, A549, and HEK293, where wild-type, mutant, and null p53 status are routinely characterized. The unconjugated format leaves detection chemistry entirely in the researcher's hands, and the 100 µl pack size keeps consumption modest for pilot studies. For best results, the liquid should be aliquoted to limit freeze-thaw cycles and protected from light when paired with fluorescent secondaries. Intended for research use only in vitro and in vivo model systems, the reagent supports assays including western blot analysis of p53 stabilization following DNA damage, IHC evaluation of p53 accumulation in tumor sections, and immunofluorescence imaging of nuclear p53 localization.