Anti-TP53 Antibody

Supporting cancer biology and stress response studies, this unconjugated Rabbit polyclonal IgG reagent from Antibodies.com targets the tumor suppressor protein p53, encoded by the TP53 locus on chromosome 17p13.1. The antibody is supplied as a 200µl liquid aliquot and is validated for Western blot (WB) and immunofluorescence (IF) workflows, with confirmed cross-reactivity to human, mouse, and rat samples.

As a sequence-specific transcription factor, p53 becomes stabilized and activated in response to cellular stress events such as DNA damage, oncogene activation, and hypoxia. Researchers use this Antibodies.com reagent to monitor endogenous p53 expression levels, map subcellular localization in fixed cells, and probe stress-signaling pathways relevant to cancer research and apoptosis regulation. The polyclonal format enables recognition of multiple epitopes on the p53 protein, which is advantageous when probing truncated isoforms or mutant variants frequently observed in tumor-derived samples.

The unconjugated IgG format allows flexibility to pair the antibody with user-selected fluorophore or enzyme conjugates, supporting both fluorescent imaging and chromogenic detection strategies. The 200µl pack size accommodates routine western blot and immunofluorescence assays, and the cross-reactivity profile across three common model species supports comparative oncological studies. Researchers commonly deploy this reagent to investigate genotoxic stress responses, cellular senescence, and tumor suppressor pathway activity in cell lines and primary culture systems.