Anti-TP53 Antibody

This polyclonal primary antibody, manufactured by Antibodies.com, targets the TP53 tumor suppressor protein and is raised in rabbit to support laboratories investigating cancer biology, DNA damage signaling, and apoptosis. Validated for Western blot and immunofluorescence workflows, the reagent detects TP53 in samples derived from human, mouse, and rat sources, covering the three species most commonly used in translational tumor research. Supplied as 100 µl of unconjugated IgG, the antibody gives researchers the freedom to pair it with HRP-conjugated, biotinylated, or fluorophore-labelled secondary reagents of their own choice, which is convenient when integrating p53 detection into multiplexed immunofluorescence panels alongside other markers.

Because TP53 mutations underpin genomic instability and the G1/S cell-cycle checkpoint response, this reagent is frequently used to probe transformed and primary cell lines for total p53 expression, post-translational modification, and stress-induced accumulation following genotoxic treatment. The unconjugated format also lends itself to immunoprecipitation protocols and to downstream applications where a directly conjugated primary would otherwise be limiting. Researchers handling the vial should adopt standard cold-chain practice: store refrigerated between uses, minimize freeze-thaw cycles, and aliquot upon first thaw to preserve IgG binding activity over the working shelf life of the reagent.