Anti-TOPBP1 Antibody

Rabbit polyclonal anti-TOPBP1 antibodies from Antibodies.com are raised against human TOPBP1, a multifunctional scaffold protein that sits at the intersection of DNA replication initiation and the DNA damage response. TOPBP1 coordinates checkpoint signaling by engaging the ATR kinase pathway through its tandem BRCT domains, which makes this reagent a frequent tool in studies of replication stress, genome stability, and oncogenesis.

Supplied as 100 µL of unconjugated polyclonal antiserum, the antibody is validated for western blot, immunohistochemistry, and immunofluorescence on Human samples. Investigators apply it to track endogenous TOPBP1 levels in cell lysates, visualize its recruitment to stalled replication forks by IF, and chart its expression patterns across tissue sections by IHC. Storage at -20 °C in small aliquots helps preserve activity across multiple freeze-thaw cycles for ongoing replication and checkpoint projects.