Anti-TOP2A Antibody

Raised in rabbit and supplied as an unconjugated polyclonal reagent from Antibodies.com, this antibody targets DNA topoisomerase II alpha (TOP2A), a nuclear enzyme essential for resolving DNA supercoiling and decatenation during replication, transcription, and mitotic chromosome segregation. Validated for western blot, immunohistochemistry, and immunofluorescence applications on human samples, the 100 µl formulation supports multiple experimental setups in tumor biology and cell cycle research. Because TOP2A expression rises sharply in proliferating cells, this reagent is widely used as a proliferation marker in studies of breast carcinoma, glioma, and other solid tumors, where amplification or overexpression of the TOP2A gene is correlated with aggressive disease and response to topoisomerase-inhibitor chemotherapy such as etoposide or doxorubicin. Researchers investigating DNA damage response, chromosomal instability, or predictive biomarker panels that include HER2 and Ki-67 alongside TOP2A will find this antibody useful for detecting endogenous protein in cultured cell lines, formalin-fixed paraffin-embedded tissue sections, and immunocytochemistry preparations.