Anti-TOP1 Antibody

Rabbit polyclonal antibody targeting DNA topoisomerase I (TOP1), the nuclear enzyme that relieves torsional stress in duplex DNA through transient single-strand cleavage and religation. This unconjugated IgG reagent is validated for western blot, immunohistochemistry, and immunoprecipitation workflows on human and mouse samples, supporting research into replication fork progression, transcription dynamics, and the cellular response to camptothecin-class chemotherapeutics such as topotecan and irinotecan that trap the covalent TOP1-DNA cleavage complex. Supplied by Antibodies.com as a 50 µl vial of polyclonal sera, the antibody provides a practical tool for studies of chromatin topology, replication stress signaling, and oncology biomarker development where TOP1 expression levels correlate with drug sensitivity in colorectal, ovarian, and small-cell lung tumor models. The antibody is raised in rabbit and is suitable for readouts that track enzyme abundance, subnuclear localization, and association with chromatin-bound partners in basic and translational research use only applications.