Anti-TNNI3 Antibody

From the Antibodies.com catalogue, this primary antibody is a Rabbit polyclonal reagent that targets TNNI3, also known as Cardiac Troponin I. It is of the IgG isotype, supplied unconjugated, and validated for Western Blot (WB) and Immunohistochemistry (IHC) use, with the manufacturer additionally listing WB and ICC/IF among its validated applications. The antibody reacts with Human, Mouse, and Rat samples.

It is raised against a recombinant fusion protein corresponding to amino acids 1–210 of human Cardiac Troponin I (TNNI3), referenced under accession NP_000354.4. The reported molecular weight is 26 kDa, and the antibody is supplied as a liquid in Phosphate Buffered Saline (pH 7.3) containing 50% glycerol and 0.02% sodium azide, with a lot-specific concentration. It has been affinity purified. Recommended working dilutions are WB 1:500–1:2,000 and ICC/IF 1:50–1:200. The product is shipped at 4°C and should be aliquoted and stored at -20°C, avoiding repeated freeze/thaw cycles.