Anti-TNFSF4 Antibody

This rabbit polyclonal antibody from Antibodies.com targets TNFSF4, the TNF superfamily member also known as OX40L or CD252, a type II transmembrane glycoprotein that serves as the ligand for the costimulatory receptor OX40 (CD134) on activated T cells. The antibody is supplied as a 100 µl aliquot at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide as preservative, with the working formulation adjusted to approximately pH 7.3.

Validated for Western blot detection, the unconjugated reagent is raised in rabbit and cross-reacts with TNFSF4 from human, mouse, and rat origin, supporting translational studies that span rodent models and human tissue samples. The polyclonal clonality means the reagent recognizes multiple epitopes across the target, which can be helpful when working with denatured protein preparations on immunoblotting membranes.

For handling, refrigerate the vial upon arrival and protect from light; the sodium azide component inhibits microbial growth, so the reagent is ready to use after a brief equilibration to room temperature. Dilute into standard blocking buffer or use neat where high-titre detection is needed. As a Primary Antibody reagent, it is intended for research use only and is compatible with standard anti-rabbit IgG secondary antibodies conjugated to HRP, fluorophores, or alkaline phosphatase.

Researchers studying costimulatory signalling, T-cell activation, autoimmune disease models, transplant rejection, and tumour immunology frequently use TNFSF4/OX40L detection by Western blot, and this Antibodies.com reagent fits workflows that look at protein-level expression of the ligand in lymphoid tissues, endothelial cells, and activated antigen-presenting cells.