Anti-TNFSF14 Antibody

The Antibodies.com anti-TNFSF14 polyclonal antibody is raised in rabbit against the TNF superfamily member 14 (LIGHT) cytokine and is supplied at 1 mg/ml in PBS containing 0.05% sodium azide at approximately pH 7.3. Presented as an unconjugated 100 µl volume, the reagent is validated for Western blot detection of the TNFSF14 target in human and mouse samples. LIGHT (also designated CD258 or HVEM-L) functions as a costimulatory cytokine that engages the HVEM receptor on T lymphocytes as well as the LTβR, influencing lymphoid tissue organization, T cell activation, and downstream inflammatory signaling through NF-κB. Researchers investigating immune checkpoint pathways, tumor microenvironment crosstalk, or chronic inflammatory disease models use this reagent to probe endogenous LIGHT expression by Western blot in lysates from activated immune cell cultures and transfected expression systems. The polyclonal format captures multiple epitopes across the TNFSF14 extracellular region, supporting robust signal across denaturing gel conditions. The buffer composition is compatible with standard SDS-PAGE sample preparation, though the presence of sodium azide should be noted for downstream live-cell or enzyme-conjugation workflows. For research use only.