Anti-TNFAIP6 Antibody

The TNFAIP6 protein, also called TSG-6 or Tumor Necrosis Factor Alpha Induced Protein 6, is a secreted hyaluronan-binding glycoprotein that participates in extracellular matrix remodeling, leukocyte trafficking, and the resolution of inflammation. This unconjugated rabbit polyclonal reagent from Antibodies.com was raised against TNFAIP6 and is validated for Western blot and immunohistochemistry workflows using human, mouse, and rat samples, supporting studies of mesenchymal stem cell paracrine activity, ovarian cumulus expansion, and chronic inflammatory disease models such as rheumatoid arthritis and lung injury.

The antibody ships as 100 µl of liquid at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, supplied in a vial ready for dilution into standard blocking buffers. Aliquot upon arrival and store at -20 °C to limit freeze-thaw cycles, keeping the working stock refrigerated between uses. Because the product is unconjugated, investigators can pair it with their own HRP, biotin, or fluorophore-labeled secondary reagents to fit chemiluminescent, chromogenic, or immunofluorescence detection platforms.

In Western blot, the reagent detects endogenous TNFAIP6 in lysates of TNF-α or IL-1β stimulated fibroblasts, macrophages, or mesenchymal stem cells, where the protein is strongly induced. For immunohistochemistry, the polyclonal format provides broad epitope coverage and is applied to formalin-fixed paraffin-embedded sections of inflamed synovium, ovarian follicle, and tumor stroma. Cross-reactivity is confirmed for Human, Mouse, and Rat, while reactivity in other species has not been formally established.