Anti-TNF-R1 Antibody

Raised in rabbit and affinity-purified against a recombinant human TNF-R1 immunogen, this polyclonal reagent from Antibodies.com is designed to detect the endogenous total pool of TNF-R1 (also known as TNFRSF1A, TNFR-I, TNFAR, p55, or tumor necrosis factor receptor type I). The unconjugated antibody is supplied at a working concentration of 1.0 mg/mL in a 50 µl volume, formulated in phosphate-buffered saline (without Mg²⁺ and Ca²⁺) at pH 7.4 with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a cryoprotectant. It is shipped on cold packs at 4 °C and should be aliquoted upon arrival and stored at –20 °C, with repeated freeze/thaw cycles avoided to preserve binding activity. Validated applications include Western blot (WB), immunohistochemistry (IHC), and immunocytochemistry/immunofluorescence (ICC/IF), and the antibody has been confirmed to cross-react with Human, Mouse, and Rat samples, making it suitable for comparative studies across the three most commonly used model systems. The reagent is intended for research use only and is not validated for diagnostic or therapeutic applications; laboratories performing western detection can expect a single band corresponding to the ~55 kDa TNF-R1 receptor, while IHC and ICC/IF users benefit from the polyclonal response, which increases the likelihood of robust signal even when antigen accessibility is limited by fixation. For ordering, the 50 µl format is stocked alongside a larger 100 µl option, and bulk or custom aliquot requests can be accommodated through the supplier's catalog listing.