Anti-TNF alpha Antibody [B1E4]

Mouse monoclonal IgG1 antibody from Antibodies.com targets human Tumor Necrosis Factor alpha (TNF-α), a pro-inflammatory cytokine central to immune regulation and the pathogenesis of inflammatory and autoimmune conditions including rheumatoid arthritis, inflammatory bowel disease, and psoriasis.

The B1E4 clone, biotinylated for sensitive streptavidin-based signal amplification, is supplied as 100 µl of liquid reagent for ELISA workflows measuring cytokine release in research samples. TNF-α, also called cachectin or TNFSF2, is initially expressed as a 26 kDa transmembrane precursor and cleaved by TACE/ADAM17 to release the soluble 17 kDa trimeric cytokine that signals through TNFR1 and TNFR2 receptors on target cells.

Researchers investigating septic shock, cytokine storms, host-pathogen responses, or screening anti-inflammatory therapeutic candidates can apply the biotinylated antibody to antigen-coated plates, then develop with streptavidin-HRP or streptavidin-fluorophore conjugates for a quantitative read-out. Aliquot upon receipt to limit freeze-thaw cycles, protect from light to preserve biotin activity, store refrigerated for active use, and freeze the vial for long-term storage.