Anti-TNF alpha Antibody [B1E4]

This mouse monoclonal antibody, clone B1E4, targets human Tumor Necrosis Factor alpha (TNF alpha), a pro-inflammatory cytokine central to immune regulation, acute-phase responses, and apoptotic signaling. Produced by Antibodies.com as a ready-to-use HRP conjugate, the reagent removes the need for a labeled secondary antibody in plate-based immunoassay workflows.

Supplied as a 200 µl liquid IgG1 preparation, the antibody is validated for ELISA and supports quantification of TNF alpha released from activated macrophages, monocytes, and stimulated cell lines. Investigators of cytokine storm biology, autoimmune disease models, rheumatoid arthritis pathways, inflammatory bowel disease, and sepsis-related signaling cascades apply the B1E4 reagent when measuring secreted TNF alpha in culture supernatants and conditioned media.

Direct HRP labeling streamlines plate protocols and reduces background associated with secondary detection steps. Monoclonal consistency ensures defined epitope specificity, supporting reproducible quantification across repeated experiments and longitudinal sample sets.