Anti-TMS1 Antibody

Raised in rabbit against the TMS1 target (also referred to as ASC or PYCARD), this polyclonal antibody from Antibodies.com supports detection of the inflammasome adaptor in both human and rat samples. The unconjugated reagent is supplied as 50 µl of a 1 mg/ml stock in PBS with 0.1% sodium azide and 50% glycerol, giving a flexible working concentration for a range of immunodetection protocols.

Validated applications include Western blot, immunohistochemistry, and immunofluorescence, covering workflows from cell lysate analysis to fixed-tissue and cultured-cell imaging. The polyclonal format means the reagent recognizes multiple epitopes on TMS1, which can be advantageous when probing endogenous expression levels in primary cells and tissue sections where single-clone reagents may give marginal signal.

Store the vial at -20 degrees C in single-use aliquots to limit freeze-thaw cycles and protect the antibody from contamination. The glycerol-containing buffer prevents ice-crystal damage during frozen storage. For immunohistochemistry on paraffin sections, heat-induced antigen retrieval with citrate buffer (pH 6.0) is widely used, while immunofluorescence staining on adherent cells can be performed following standard paraformaldehyde fixation and permeabilization with 0.1% Triton X-100. Brief centrifugation before opening recovers any antibody that may have condensed near the cap.