Anti-TKT Antibody

Transketolase (TKT) is a thiamine-dependent enzyme that catalyzes key carbon-transfer reactions in the non-oxidative branch of the pentose phosphate pathway, connecting glycolytic flux with NADPH production and ribose-5-phosphate generation for nucleotide biosynthesis. This rabbit polyclonal antibody from Antibodies.com is raised against TKT and validated for western blot and immunofluorescence workflows, supporting detection of the endogenous protein in human, mouse, and rat samples. The reagent is supplied as 100 µL of unconjugated antiserum, allowing flexible pairing with secondary reporters such as HRP- or fluorophore-conjugated antibodies and custom labeling strategies. Investigators studying cancer metabolism, thiamine deficiency models, and redox homeostasis commonly probe TKT expression to map pentose phosphate pathway activity in cell lines and tissue sections. Each lot is affinity-purified and quality-tested to deliver reproducible band patterns on immunoblots and clean cytoplasmic staining in immunofluorescence imaging. The polyclonal format increases the likelihood of recognizing multiple epitopes, which is useful when probing conserved regions of transketolase across species or when working with post-translationally modified targets. Handle the vial on ice during use and aliquot if needed to limit freeze-thaw cycles that can degrade polyclonal activity over time.