Anti-TIMP-2 Antibody

Raised in rabbit against tissue inhibitor of metalloproteinases 2 (TIMP-2), this unconjugated polyclonal reagent from Antibodies.com is supplied as 100 µl of ready-to-use antiserum suitable for western blot, immunohistochemistry, immunofluorescence, and ELISA workflows. The antibody recognizes an epitope conserved across human, mouse, and rat TIMP-2, supporting comparative studies of extracellular matrix remodeling, MMP pathway regulation, and cell-cycle signaling research.

TIMP-2 is a secreted glycoprotein of approximately 24 kDa that broadly inhibits matrix metalloproteinases and uniquely participates in MT1-MMP-mediated activation of pro-MMP-2, giving it both suppressive and activating roles in collagen turnover. Because it is a polyclonal reagent, the antiserum binds multiple epitopes on the target, which is often advantageous when probing denatured samples on membranes or fixed tissue sections where conformational epitopes may be compromised. Researchers working on tumor invasion, fibrotic disease models, or the link between circulating TIMP-2 and hippocampal function in aging studies will find the broad species reactivity useful for validating findings across mouse and human samples.

Store the vial at -20 °C in small aliquots to minimize freeze-thaw cycles and protect from light; for short-term use, refrigeration at 4 °C is acceptable for working stocks. The unconjugated format allows downstream pairing with HRP, fluorophore, or biotin secondary reagents of the researcher's choice, supporting multiplex labeling strategies in immunofluorescence and chromogenic detection in IHC.