Anti-TIF1α Antibody

This polyclonal antibody from Antibodies.com is raised in rabbit against human TIF1α (also known as TRIM33 or RFG7), a member of the tripartite motif family of proteins. The unconjugated reagent is supplied at 100 µl volume and is validated for western blot, immunohistochemistry, and ELISA workflows, with reactivity confirmed against human and mouse samples. TIF1α functions as an E3 ubiquitin ligase within the TGF-β signaling cascade and participates in transcriptional regulation through chromatin remodeling at target promoters. Researchers studying SMAD4-dependent signaling, innate immune regulation, DNA damage response, and myeloproliferative neoplasms use anti-TIF1α antibodies to probe ubiquitination events and nuclear body formation. Polyclonal recognition of multiple epitopes can be advantageous when monitoring expression changes, post-translational modifications, or isoform-specific patterns in cell lysates and tissue sections. The unconjugated format allows end users to conjugate the antibody to their preferred reporter label, supporting immunofluorescence and chromogenic detection workflows in cell biology and oncology research.