Anti-Thyroxine (T4) Antibody

Raised in rabbits and purified by Protein A affinity chromatography, this unconjugated polyclonal antibody from Antibodies.com selectively binds thyroxine (T4), the predominant circulating thyroid hormone synthesized by the thyroid gland. The reagent is supplied in a 1 mg vial as a lyophilized powder and is validated for ELISA workflows in endocrine and metabolic research.

Anti-T4 antibodies are widely applied to investigate thyroid axis function, including characterization of hormone secretion patterns in rodent disease models, screening of serum and culture supernatant for hormone levels, and analysis of binding interactions with carrier proteins such as thyroxine-binding globulin, transthyretin, and albumin. The unconjugated format gives researchers flexibility to conjugate the antibody to HRP, biotin, or fluorophores for tailored assay architectures, and the polyclonal nature of the reagent supports recognition of multiple epitopes across the T4 molecule.

For short-term use, store the antibody refrigerated at 4 degrees C; for long-term storage, freeze aliquots at -20 degrees C or below and minimize freeze-thaw cycles to preserve activity. Working dilutions, coating concentrations, and incubation times should be optimized for each ELISA plate format and matched to the chosen detection substrate.