Anti-TH (phospho S40) Antibody

Selective detection of tyrosine hydroxylase phosphorylated at serine 40 (pSer40) is essential for mapping kinase-driven control of catecholamine biosynthesis in dopaminergic and noradrenergic neurons. This rabbit polyclonal reagent from Antibodies.com was raised against a synthetic phosphopeptide centered on the R-Q-S(p)-L-I motif surrounding the phospho-Ser40 site, and is supplied as an unconjugated IgG in a 100 µl aliquot that is affinity-purified for use in immunofluorescence workflows on human, mouse, and rat specimens.

Researchers apply the antibody to investigate dopamine synthesis regulation in cell culture and tissue sections, including PC-12 chromaffin cells and midbrain neuron models relevant to Parkinson's disease, addiction circuitry, and stress-response biology. Indirect immunofluorescence at working dilutions around 1:1,000 produces clean labeling of the ~60 kDa TH band in catecholaminergic cell bodies and fibers, while pre-adsorption with the corresponding non-phosphopeptide abolishes signal and confirms phospho-specificity. The reagent detects only the phosphorylated form and does not bind unmodified TH.

The product is intended for research use only. For best stability, store the vial refrigerated, avoid repeated freeze-thaw cycles, and protect from light to preserve phospho-specific binding activity over the shelf life of the aliquot.