Anti-TGM5 Antibody

Transglutaminase 5 (TGM5) is a calcium-dependent cross-linking enzyme expressed primarily in epidermal keratinocytes, where it contributes to cornified cell envelope formation and the mechanical barrier function of the skin. The Anti-TGM5 Antibody from Antibodies.com is a rabbit polyclonal reagent that detects endogenous TGM5 protein in human and mouse samples, supporting dermatology and skin biology research including studies of acral peeling skin syndrome (APSS), a TGM5 mutation-linked genodermatosis characterized by painless superficial peeling of the epidermis.

This unconjugated primary antibody is supplied in a 100 µl vial and is validated for Western Blot (WB) and Immunohistochemistry (IHC) applications. Polyclonal reactivity across multiple epitopes typically enhances signal recovery in tissues where TGM5 expression is moderate or focal. Researchers can apply the reagent to characterize TGM5 distribution across stratified epithelium, patient-derived skin biopsies, or relevant mouse models of barrier dysfunction.

For Western Blot, the antibody is compatible with standard HRP- or fluorescence-conjugated secondary reagents and resolves the protein at its expected molecular weight. For IHC on formalin-fixed paraffin-embedded sections, antigen retrieval followed by chromogenic or fluorescent detection reveals TGM5 localization in the upper epidermal layers. Storage and handling follow standard antibody practices: aliquot upon arrival and store refrigerated to preserve activity across multiple freeze-thaw cycles.