Anti-TGF beta Antibody [2C5]

Mouse monoclonal antibodies targeting Transforming Growth Factor beta are central reagents in cytokine-signaling research, and this unconjugated IgG1 reagent from Antibodies.com, raised as clone 2C5 in mouse, is supplied as 100 µg of lyophilised powder for reconstitution in laboratory buffer. The unconjugated format lets researchers couple the antibody to horseradish peroxidase, biotin, or fluorescent dyes for their own detection chemistries, while the lyophilised presentation supports long-term storage and convenient aliquoting to limit freeze-thaw exposure of the working stock.

TGF beta is a pleiotropic cytokine that regulates proliferation, differentiation, apoptosis, and extracellular matrix deposition through SMAD2/3-dependent and SMAD-independent cascades. Antibodies raised against the ligand are routinely used to confirm conditioned-medium activity, profile autocrine and paracrine signaling in vitro, and characterize fibrotic, inflammatory, or tumor-microenvironment models in vivo. The antibody shows reactivity with human TGF beta and is validated for ELISA and western blot workflows, supporting quantification of secreted cytokine in cell-culture supernatants and detection of the mature active form in cell lysates.

Reconstitution with sterile buffer, storage at the temperature indicated on the accompanying datasheet, and aliquoting to avoid repeated freeze-thaw cycles preserve binding activity. Researchers investigating epithelial-mesenchymal transition, regulatory T-cell function, idiopathic pulmonary fibrosis, or hepatocellular carcinoma will find the 2C5 clone a useful addition to their antibody panel, complementing other Primary Antibodies used in signaling-pathway dissection, immunohistochemistry-adjacent studies, and downstream flow cytometry when conjugated by the end user.