Anti-TFIIB Antibody

This mouse monoclonal IgG2b antibody from Antibodies.com targets Transcription Factor II B (TFIIB), a general transcription factor required for accurate initiation by RNA polymerase II. TFIIB stabilizes the pre-initiation complex by bridging the TATA-binding protein (TBP)–TATA element assembly with Pol II and helps define the transcription start site through its B-reader helix.

Validated for western blot (WB), immunocytochemistry (ICC), and immunohistochemistry (IHC), the unconjugated reagent ships as 100 µl of liquid stock. Reactivity spans Human, Mouse, Rat, and Canine samples, supporting comparative work across standard mammalian model systems and canine-derived tissue or cell lines.

Because TFIIB abundance reflects the status of the basal transcriptional machinery, this clone is used to investigate Pol II promoter engagement, transcriptional dysregulation in cancer, and changes in initiation during differentiation and cellular stress. Store the vial refrigerated at 4°C; for longer-term storage, freeze aliquots at -20°C and avoid repeated freeze-thaw cycles of the working stock.