Anti-TFF2 Antibody

Antibodies.com supplies this rabbit polyclonal antibody raised against Trefoil Factor 2 (TFF2), a secreted mucin-associated peptide of the trefoil factor family that protects gastrointestinal epithelia and supports mucosal repair after injury. The unconjugated reagent is provided as a 100 µl vial at 1 mg/ml in Phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.3, and the antibody has been validated for Western blot on Human, Mouse, and Rat samples.

TFF2 is normally secreted by mucous neck cells and pyloric gland cells of the stomach as well as Brunner's glands of the duodenum. Because expression is dramatically upregulated in spasmolytic polypeptide-expressing metaplasia (SPEM), a preneoplastic lesion linked to chronic atrophic gastritis, this reagent is widely used to score gastric injury models and to track gastric cancer progression in research settings. Western blot detection of the mature TFF2 peptide resolves a band of approximately 12 kDa when stomach or intestinal lysates are run under reducing conditions.

For handling, the vial should be stored at -20 °C in single-use aliquots and protected from light; freeze-thaw cycles should be minimized to preserve binding activity. The polyclonal format offers recognition of multiple epitopes across the trefoil P-domain, which improves detection of partially degraded or truncated forms encountered in tissue lysates. Research applications include studies of mucosal inflammation, Helicobacter pylori pathogenesis, and Barrett's esophagus-associated metaplasia, where TFF2 induction is monitored in response to injury or pharmacological challenge.