Anti-TFAM Antibody

TFAM (Transcription Factor A, Mitochondrial) is a nuclear-encoded regulator of mitochondrial DNA transcription, replication, and nucleoid packaging, and this rabbit polyclonal primary antibody enables its detection in cell lysate and tissue homogenate samples. The unconjugated reagent from Antibodies.com is supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.3, with each vial containing 100 µl of working solution.

Validated for Western blot applications, the antibody cross-reacts with human, mouse, and rat TFAM, supporting comparative mitochondrial studies across common model organisms. Typical investigations include mitochondrial biogenesis, mtDNA copy number dynamics, oxidative phosphorylation capacity, and cellular responses to metabolic or hypoxic stress. Researchers have applied TFAM immunoblotting to characterize mtDNA depletion syndromes, exercise-induced mitochondrial adaptations, and alterations observed in cardiac or neuronal tissue under pathological conditions.

For routine use, store the vial refrigerated at 4 degrees C; aliquot and freeze for long-term storage, and minimize freeze-thaw cycles to preserve binding activity. The included 0.05% sodium azide serves as a preservative against microbial contamination during repeated pipetting sessions.