Anti-TF3B Antibody

Rabbit polyclonal antibody from Antibodies.com raised against the TF3B target and supplied as 100 µl of unconjugated antiserum. Validated for western blot, immunohistochemistry, and immunofluorescence workflows on human samples, this Primary Antibody reagent gives investigators flexibility to choose their own detection chemistry.

Polyclonal preparations contain a heterogeneous population of immunoglobulin molecules that recognize multiple epitopes on the same antigen, an advantage when target expression is variable across samples or when individual binding sites are partially masked by fixation or post-translational modification. Because the antibody is unconjugated, it is paired with a secondary reagent of the researcher's choosing: HRP-conjugated secondaries support chromogenic western blot and immunohistochemistry, while fluorophore-labeled secondaries enable immunofluorescence detection on fixed cells, allowing same-lot comparisons across imaging and blotting readouts.

Typical use involves SDS-PAGE separation of lysates followed by transfer to a membrane for western blot, antigen retrieval on fixed tissue sections for immunohistochemistry, and standard fixation and permeabilization for immunofluorescence. The product is shipped as a single vial and should be refrigerated upon arrival; for long-term storage, aliquot to limit freeze-thaw cycles and protect from light if downstream detection involves fluorescent conjugates.