Anti-TET2 (di methyl R1682) Antibody

The TET2 dioxygenase catalyzes the oxidation of 5-methylcytosine to 5-hydroxymethylcytosine in DNA, positioning it as a central regulator of active demethylation and epigenetic reprogramming in mammalian genomes. This rabbit polyclonal IgG reagent from Antibodies.com was raised against a synthetic peptide carrying di-methyl arginine 1682 (R1682), enabling selective detection of the post-translationally modified form of TET2 rather than the total protein pool.

Supplied as 100 µl of unconjugated antiserum, the antibody is validated for immunofluorescence workflows on human samples, where it produces nuclear-localized signal consistent with the established chromatin association of TET2. The polyclonal response targets multiple epitopes surrounding the dimethyl-R1682 mark within the catalytic domain, which reduces dependence on any single linear peptide and supports clear visualization across IF preparations, including confocal imaging of fixed cell monolayers and tissue cryosections.

Investigators apply the reagent to fixed cells or tissue sections together with an anti-rabbit secondary fluorophore; working dilutions should be titrated empirically against each sample preparation and detection system to balance signal against background in immunofluorescence. Because the antibody distinguishes the di-methyl R1682 state from the unmodified peptide, it is suited for studies of post-translational regulation of TET2 activity, including investigations in hematopoietic differentiation, myelopoiesis, and clonal hematopoiesis where TET2 dysregulation is recurrently observed. Each vial is intended for research use only.