Anti-Testosterone Antibody

Produced by Antibodies.com as a Protein G purified preparation, this unconjugated mouse monoclonal IgG2b antibody specifically recognizes testosterone. The reagent is supplied at 1 mg total protein and is formatted for ELISA workflows as well as other immunoassay applications.

The antibody is raised in mouse hosts and isolated through Protein G affinity chromatography, yielding an unconjugated IgG2b isotype that can be labeled in-house with HRP, biotin, fluorophores, or other reporters according to the chosen detection scheme. The defined IgG2b subclass supports consistent secondary antibody selection and isotype-matched control design during assay optimization.

For competitive ELISA, the antibody is typically paired with coated antigen or conjugated tracer to quantify testosterone in diluted samples. As a capture reagent in two-site assays, it can be immobilized directly onto microplate wells. Titration against a calibrated antigen standard is recommended to determine the optimal working concentration for each plate format and matrix.

To preserve activity, the lyophilized or reconstituted antibody should be aliquoted into single-use vials, stored frozen for long-term stability, and protected from repeated freeze-thaw cycles. Working dilutions kept at refrigerated conditions are stable for limited periods. As with any primary antibody, end users are responsible for validating compatibility with their specific sample matrix and for characterizing cross-reactivity against related steroid analytes as part of routine assay qualification.