Anti-TDP2 Antibody

TDP2 (Tyrosyl-DNA Phosphodiesterase 2) is a nuclear enzyme that hydrolyzes 5'-phosphotyrosyl bonds to resolve covalent topoisomerase II-DNA complexes, a key step in repairing DNA damage caused by topoisomerase II-targeting agents such as etoposide. This polyclonal reagent from Antibodies.com is raised in rabbit against TDP2 and validated for western blot and immunohistochemistry workflows, with reactivity confirmed on both human and mouse samples.

Supplied as 100 µl of unconjugated antibody, the reagent is intended for research use only. The unconjugated format lets researchers pair it with HRP-, fluorophore-, or biotin-conjugated secondary antibodies, depending on whether the downstream read-out is chemiluminescent western blot or chromogenic or fluorescent IHC on fixed tissue sections. It performs reliably on whole-cell lysates and on paraffin-embedded sections, enabling detection of endogenous TDP2 across a range of cell and tissue models.

Typical applications include probing TDP2 expression changes after topoisomerase II inhibition, comparing repair capacity in tumor versus normal tissue, and localizing the protein in neural and other tissues where TDP2 function has been linked to genome stability and chemosensitivity. Because the antibody recognizes both human and mouse orthologs, it supports parallel studies in patient-derived samples and corresponding murine models of DNA damage and chemosensitivity.