Anti-TCEB3 Antibody

A polyclonal antibody raised in rabbit is offered by Antibodies.com. It is described as a rabbit polyclonal antibody to TCEB3 (also referred to as Elongin-A) and is supplied as an unconjugated IgG preparation in liquid form. According to the product specification sheet, it is listed for use in Western blot (WB) and immunofluorescence (ICC/IF) applications, with reactivity reported against human, mouse, and rat samples. The manufacturer's catalogue entry lists WB, IHC, and IF under applications. The immunogen is a recombinant fusion protein corresponding to amino acids 679–798 of human TCEB3 (NP_003189.2). Recommended dilutions given are WB at 1:500–1:2,000 and ICC/IF at 1:50–1:200.

The antibody is purified by affinity chromatography and is supplied in Phosphate Buffered Saline (pH 7.3) containing 50% glycerol and 0.02% sodium azide. It is shipped at 4 °C and should be aliquoted and stored at –20 °C upon arrival, with freeze/thaw cycles to be avoided. The reported molecular weight of the target is 105 kDa. The product is for research use only and is not intended for diagnostic or therapeutic applications.

Supporting images include a Western blot of extracts from various cell lines using the antibody at 1:1,000 dilution, with a goat anti-rabbit IgG H&L (HRP) secondary at 1:10,000, lysates at 25 µg per lane, 3% non-fat dry milk in TBST as blocking buffer, ECL Enhanced Kit (RM00021) detection, and a 30 s exposure. An immunofluorescence image of MCF7 cells using the antibody is also shown.