Anti-TB 38Kd Antigen Antibody [B12F8]

The 38 kDa antigen (also known as PhoS or PstS1) is an immunodominant Mycobacterium tuberculosis lipoprotein that is widely investigated as a target for serological TB research and vaccine development. Antibodies.com offers this mouse monoclonal reagent, clone B12F8, which binds a defined epitope on the antigen and supports detection in ELISA and western blot workflows. The antibody is supplied as 100 µg of lyophilised IgG1 in unconjugated format, providing flexibility for downstream labelling with HRP, biotin, or fluorophores depending on the assay design. Reconstitution in PBS yields a stable stock suitable for refrigerated storage and direct use in antigen-coating, capture, or detection layers. Because the antigen is conserved across M. tuberculosis complex strains, the reagent is applied to studies of humoral immune responses in patient cohorts, screening of recombinant vaccine candidates, and characterisation of TB diagnostic antigens. Researchers typically titrate the antibody empirically for each application, with dilutions in the low microgram-per-millilitre range commonly reported for both ELISA plate coating and western blot detection. Each lot is validated for specificity against the recombinant antigen, minimising cross-reactivity in routine laboratory use.