Anti-Tau (phospho Thr181) Antibody [9G4]

The 9G4 monoclonal antibody from Antibodies.com is a mouse-derived IgG1 reagent raised against the Tau protein phosphorylated at threonine 181, a residue implicated in paired helical filament formation and early Alzheimer's disease pathology. Supplied as 100 µg of lyophilised product, it is validated for Western Blot detection of phospho-Thr181 Tau in human and mouse sample preparations, allowing researchers to probe tau hyperphosphorylation events in neuronal cell lysates and brain tissue homogenates.

Because the phospho-Thr181 epitope appears prominently in cerebrospinal fluid and cortical extracts from subjects with mild cognitive impairment and early-stage Alzheimer's, this clone is widely used in neurobiology labs studying kinase signalling cascades involving GSK-3β and CDK5. The unconjugated format gives users flexibility to conjugate the antibody to their preferred reporter, while the lyophilised storage profile simplifies shipping and long-term bench stability when reconstituted and stored refrigerated with the recommended buffer.

Produced in mouse and raised against a phosphorylated peptide immunogen corresponding to the Thr181 region of human Tau, the 9G4 clone distinguishes the phosphorylated state from unphosphorylated tau, an important control when interpreting western blot banding patterns across treatment conditions. Researchers handling transgenic mouse models, post-mortem human brain specimens, or tauopathy cell culture systems commonly rely on this specificity to confirm pathway activation downstream of amyloid exposure, oxidative stress, or pharmacological challenge. Each lot is supplied with documentation supporting reactivity with both Human and Mouse samples and outlining recommended blocking, primary incubation, and chemiluminescent detection conditions for reproducible western blot results.