Anti-TAGLN2 Antibody

From Antibodies.com, this is a rabbit polyclonal antibody raised against TAGLN2 (Transgelin-2). The unconjugated IgG reagent is supplied as a liquid in Phosphate Buffered Saline at pH 7.3, with 50% glycerol. Preservative additives vary by formulation and include 0.02% sodium azide, 0.05% sodium azide or 0.01% thiomersal. It is shipped at 4°C for long-term storage at -20°C, with avoidance of freeze/thaw cycles, and is purified by affinity or antigen affinity chromatography.

Across the product range, reactivity profiles include human, mouse and rat samples, while validated applications cover Western blot (WB), immunocytochemistry/immunofluorescence (ICC/IF), immunohistochemistry (IHC) and ELISA. One variant is described as KO Validated. The immunogens used include a recombinant fusion protein corresponding to amino acids 1-199 of human TAGLN2, a fusion protein derived from internal residues of human Transgelin 2, and a recombinant fusion protein corresponding to amino acids 1-158 of human TAGLN2 (NP_003555.1). The predicted molecular weight of the target is 22 kDa.

Recommended starting dilutions reported by the manufacturer include WB at 1:500-1:2,000 and 1:500-1:1,000, ICC/IF at 1:50-1:200, and an ELISA starting concentration of 1 µg/ml. The antibody detects endogenous levels of total TAGLN2 protein.