Anti-TACC1 Antibody

Centrosomal biology, mitotic-spindle integrity, and microtubule organization during cell division are the research contexts served by this unconjugated polyclonal reagent raised in rabbit against the transforming acidic coiled-coil–containing protein 1 (TACC1). Supplied by Antibodies.com as a 100 µl volume, the antibody is validated for Western blot (WB) and immunofluorescence (IF) workflows and recognizes endogenous TACC1 in both human and mouse samples, supporting comparative studies in cancer cell biology and centrosome research.

TACC1 localizes to centrosomes throughout the cell cycle and contributes to proper spindle assembly, with altered expression observed in breast carcinoma and other solid tumor contexts where the 8p11 locus has drawn interest as a candidate biomarker. The polyclonal format allows recognition of multiple epitopes on the target, useful when working with post-translationally modified or alternatively spliced forms. Researchers studying mitotic checkpoints, chromosome segregation, or microtubule-associated protein networks can pair this reagent with chromatin-staining counterstains to map TACC1 distribution during specific phases of mitosis by IF, or resolve total protein levels in whole-cell lysates by WB.

Recommended handling: brief centrifugation before use, aliquot to reduce freeze-thaw cycles, and store at -20 °C in working volumes to preserve antibody activity. The unconjugated presentation lets each laboratory pick its preferred secondary label, from HRP-polymer reagents for chemiluminescent Western blots to Alexa Fluor conjugates for multicolor immunofluorescence imaging.