Anti-Synaptotagmin 1 (Ab-309) Antibody

This polyclonal antibody from Antibodies.com is raised in rabbit against Synaptotagmin 1 (SYT1), a synaptic vesicle integral membrane protein that serves as the principal calcium sensor for fast, synchronized neurotransmitter release. The reagent is supplied as 100 µl of unconjugated antisera and is validated for Western blot and immunofluorescence applications.

Reactive against human, mouse, and rat samples, the antibody supports investigation of presynaptic vesicle proteins in cultured neurons, brain tissue sections, and biochemical lysates. Synaptotagmin 1 contains tandem C2 domains that bind calcium and interact with SNARE complexes to trigger vesicle fusion, so this reagent is widely used in studies of synaptic transmission, vesicle cycling, and presynaptic terminal organization in neuroscience and cell biology laboratories.

For storage, refrigerate the antibody in working aliquots to limit freeze-thaw cycles, which can reduce titer over time. The unconjugated format provides flexibility to pair the antibody with standard secondary reagents for Western blot band visualization at the SYT1 molecular weight and for immunofluorescence labeling of presynaptic puncta in neuronal cultures and tissue sections.