Anti-Synaptotagmin 1 / 2 (Phospho-Thr202 / 199) Antibody

Rabbit polyclonal antibody supplied by Antibodies.com, raised against Synaptotagmin 1 / 2 when phosphorylated at threonine 202 / 199. The reagent is unconjugated and validated for immunofluorescence (ICC/IF) on Human, Mouse and Rat samples, with a recommended working dilution of 1:100 to 1:200.

The antibody was generated by immunizing rabbits with a synthetic phosphopeptide corresponding to the sequence around the threonine 202 / 199 phosphorylation site (R-K-T(p)-L-N) derived from human Synaptotagmin 1 / 2, conjugated to KLH. Purification was carried out by affinity chromatography against the epitope-specific phosphopeptide, and non-phospho-specific antibodies were removed by chromatography on the corresponding non-phosphopeptide. The product is supplied at 1.0 mg/mL in phosphate-buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol.

The antibody specifically detects endogenous levels of Synaptotagmin 1 / 2 only when phosphorylated at threonine 202 / 199, with a predicted molecular weight of 46kd. It is shipped at 4°C; upon delivery it should be aliquoted and stored at -20°C, with freeze/thaw cycles avoided.