Anti-Synaptotagmin 1 / 2 (Ab-202 / 199) Antibody

Raised in rabbit and affinity-purified over an epitope-specific peptide, this polyclonal reagent from Antibodies.com is designed to recognise endogenous levels of total Synaptotagmin 1 and Synaptotagmin 2 when phosphorylated or unmodified at the conserved threonine residue (Ab-202/199). The immunogen corresponds to the peptide sequence around amino acids 200–204 / 197–201 (R-K-T-L-N) of the human protein, and the antibody cross-reacts with Human, Mouse and Rat samples, making it a practical choice for studies spanning rodent models and human tissue. Supplied as an unconjugated liquid at 1.0 mg/mL in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol, the preparation is shipped at 4 °C from the Antibodies.com facility in Cambridge, UK, with a typical lead time of 6–9 business days. For Western blotting the recommended dilution range is 1:500–1:1000, yielding a band near the predicted molecular weight of 46 kDa, while for immunofluorescence on cultured cells or tissue sections a working dilution of 1:100–1:200 provides consistent labelling. The 50 µL pack size is sufficient for multiple routine applications and may be aliquoted and stored at –20 °C, taking care to avoid repeated freeze/thaw cycles to preserve affinity and specificity. As a research-use-only reagent, this polyclonal antibody is well suited to investigations of synaptic vesicle exocytosis, neurotransmitter release, and the broader presynaptic biology of the Synaptotagmin family.