Anti-Synaptotagmin 1 / 2 (Ab-202 / 199) Antibody

This rabbit polyclonal antibody from Antibodies.com targets Synaptotagmin 1 and Synaptotagmin 2, two closely related integral membrane proteins of synaptic vesicles that act as calcium sensors for neurotransmitter release. Raised against a synthetic peptide corresponding to residues 202/199, the reagent recognizes endogenous protein in human, mouse, and rat samples and is supplied as a 100 µl vial of unconjugated antiserum for Western blot and immunofluorescence workflows.

Synaptotagmin 1 serves as the major fast Ca2+ sensor at forebrain excitatory synapses, while Synaptotagmin 2 predominates in the hindbrain and at inhibitory terminals, making this reagent useful for tracking both isoforms in neuronal cell biology studies. Researchers apply it to label presynaptic boutons in cultured neurons, monitor synaptic vesicle recycling, and assess changes in synaptotagmin expression in disease models such as neurodegeneration and epilepsy. For western blot, the antibody is used on reduced lysates of neural tissue, and for immunofluorescence it produces a punctate staining pattern consistent with synaptic localization.