Anti-SYN1 (phospho S9) Antibody - Identical to Abcam (ab194778)

Antibodies.com offers a rabbit polyclonal antibody raised against Synapsin I phosphorylated at Ser9, supplied as a liquid reagent validated for Western blot and immunocytochemistry/immunofluorescence applications. The antibody is of the IgG isotype, is unconjugated, and reacts with human, mouse and rat samples. It was generated using a synthetic phosphorylated peptide corresponding to the region around S9 of human SYN1 (NP_008881.2), with the immunogen sequence RLSDS, and is purified by affinity chromatography.

The target protein has a molecular weight of 74 kDa. Recommended working dilutions are 1:500–1:2,000 for Western blot and 1:100–1:200 for ICC/IF. Supporting validation data include Western blot analysis of mouse brain tissue lysates at 25 µg per lane, using 3% BSA as blocking buffer and a Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at 1:10,000 dilution, together with immunofluorescence staining of methanol-fixed HeLa cells.

The reagent is provided in phosphate buffered saline (pH 7.3) containing 50% glycerol and 0.01% Thiomersal. It is shipped at 4 °C and, upon delivery, should be aliquoted and stored at −20 °C, with freeze/thaw cycles avoided. The product is intended for research use only and is not for diagnostic or therapeutic use.