Anti-SYK (phospho Y323) Antibody - Identical to Abcam (ab194852)

Antibodies.com supplies a rabbit polyclonal antibody raised against SYK phosphorylated at tyrosine 323 (phospho Y323). The unconjugated IgG reagent reacts with human samples and is validated for Western blot, immunohistochemistry, and immunofluorescence applications.

The antibody was generated using a phospho-specific peptide corresponding to residues surrounding Y323 of human Syk, and it has been affinity purified. It is provided as a liquid in phosphate buffered saline (pH 7.3) with 50% glycerol and 0.02% sodium azide, at a lot-specific concentration. The target protein, Syk, has a molecular weight of 72 kDa.

Recommended working dilutions are 1:500–1:2,000 for Western blot, 1:50–1:100 for immunohistochemistry, and 1:100–1:200 for ICC/IF. The product is shipped at 4°C; on arrival it should be aliquoted and stored at −20°C, with freeze/thaw cycles avoided. The reagent is supported by three scientific validation images.