Anti-SUPT4H1 Antibody

SUPT4H1 (Suppressor of Ty 4 Homolog 1) is the smaller subunit of the DSIF transcription elongation complex, which partners with SPT5 to control RNA polymerase II pause-release and processivity across actively transcribed genes. This rabbit polyclonal antibody from antibodies.com was raised against recombinant human SUPT4H1 and binds an epitope that is conserved in mouse and rat orthologs, as confirmed by western blot on whole-cell lysates from all three species.

The reagent is supplied as 100 µL of purified polyclonal IgG at 1 mg/ml in phosphate buffered saline (pH 7.2) with 15 mM sodium azide as a microbial preservative. The unconjugated format leaves the antibody open for direct labeling with HRP, biotin, or fluorophores, or for use in immunoprecipitation of endogenous DSIF complexes from nuclear extracts and chromatin pull-down assays that map elongation-factor occupancy at specific loci.

Common research applications include mechanistic studies of promoter-proximal pausing, investigation of SUPT4H1 involvement in repeat-associated non-AUG (RAN) translation in CAG-repeat expansion models such as Huntington's disease, and characterization of altered DSIF function in oncogenic transcription programs. For routine use, store refrigerated; for longer-term storage, aliquot the antibody and freeze at -20°C or below, avoiding repeated freeze-thaw cycles to preserve binding activity.