Anti-SUMO2 Antibody

Raised in rabbit against Small Ubiquitin-like Modifier 2 (SUMO2), this unconjugated polyclonal primary antibody from Antibodies.com is supplied as 100 µl of affinity-purified antiserum and is validated for Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) applications across human, mouse, and rat samples. SUMO2 is a ~12 kDa ubiquitin-like protein that is covalently conjugated to lysine residues on substrate proteins through the SUMOylation cascade, a post-translational modification that regulates transcription, nuclear transport, DNA repair, and the cell-cycle response to proteotoxic stress.

The antibody recognizes both free unconjugated SUMO2 and SUMO2-modified substrates, producing the characteristic high-molecular-weight conjugate smears seen in SUMOylation profiling experiments. SUMO2 is a stress-inducible member of the SUMO family and shares very high sequence identity with SUMO3, which is why most reagents, including this one, detect both paralogs. Researchers routinely apply anti-SUMO2 antibodies to monitor global SUMOylation changes following heat shock, oxidative stress, or proteasome inhibition; to map subcellular distribution of SUMO conjugates by immunofluorescence; and to identify SUMO-modified targets involved in chromatin remodeling, ribosome biogenesis, and the DNA damage response.

The polyclonal format increases the probability of recognizing multiple epitopes on SUMO2, including the N-terminal region that differs from SUMO1, while the unconjugated state allows users to couple the reagent directly to fluorophores, biotin, HRP, or gold nanoparticles depending on the chosen detection chemistry. In Western blot, the antibody detects monomeric SUMO2 at approximately 12 kDa together with high-molecular-weight SUMO2-conjugate smears. In immunohistochemistry on formalin-fixed paraffin-embedded tissue, it produces cytoplasmic and nuclear staining consistent with the broad distribution of SUMOylated proteins across cellular compartments. For immunofluorescence, it localizes to nuclear speckles, nucleoplasm, and cytoplasm in interphase cells, with redistribution to mitotic chromatin during mitosis.

This reagent is suitable for cell culture lysates, tissue homogenates, and fixed-cell imaging, and the cross-reactivity with human, mouse, and rat SUMO2 supports comparative studies between rodent disease models and clinical human samples.