Anti-SUFU Antibody

This rabbit polyclonal IgG antibody targets human SUFU (Suppressor of Fused Homolog), a key negative regulator of the Hedgehog signaling pathway that sequesters GLI transcription factors in the cytoplasm and prevents their nuclear translocation in the absence of ligand stimulation. The reagent is supplied by Antibodies.com as an unconjugated, polyclonal antibody raised in rabbit, validated for Western blot detection of endogenous SUFU protein in human samples, and is provided in a 200 µL volume suitable for multiple blotting experiments.

SUFU functions as a critical tumor suppressor in Hedgehog-driven tissues, and alterations in its expression or activity have been linked to medulloblastoma, basal cell carcinoma, and developmental disorders including Joubert syndrome. Researchers studying Hedgehog pathway regulation, GLI transcription factor biology, or cancer signaling networks use this antibody to probe SUFU expression levels, subcellular localization, and post-translational modifications in cell lysates and tissue homogenates. The unconjugated format allows flexibility in choosing secondary antibody conjugates and detection chemistries tailored to specific experimental needs.

Working aliquots help preserve antibody activity across repeated use, and brief centrifugation before each application recovers the full volume and ensures consistent concentration throughout experimental workflows.