Anti-SUFU Antibody

SUFU (Suppressor of Fused) functions as a key negative regulator of the Hedgehog signaling pathway, controlling GLI transcription factors by sequestering them in the cytoplasm. This unconjugated rabbit polyclonal IgG antibody is raised in rabbit and validated for detection of human SUFU in Western blot workflows, with a 100 µl volume providing reagent for multiple immunoblot membranes. The polyclonal response targets multiple epitopes of the protein, supporting specific signal in lysates from human cell lines and tissue samples.

Provided by Antibodies.com as a research-grade primary antibody reagent, the unconjugated format lets users select their own secondary detection system, choosing between fluorescent, chemiluminescent, or chromogenic substrates according to the experimental setup. Each preparation is supplied in a vial and supports downstream pathway readouts, including studies of Hedgehog pathway activity in development, stem cell maintenance, and oncology, where SUFU loss-of-function mutations are linked to medulloblastoma, basal cell carcinoma, and other tumor types.

Laboratory applications include investigation of GLI processing, downstream effectors such as PTCH1 and GLI1, and upstream regulatory interactions involving transmembrane proteins like Smoothened, all for research use only.