Anti-STX3 Antibody

This rabbit polyclonal primary antibody is supplied by Antibodies.com as a 100 µl vial of unconjugated IgG, affinity-purified and validated for Western blot workflows. In immunoblotting the reagent detects endogenous levels of total STX3 protein across human, mouse, and rat samples, supporting comparative analysis of native syntaxin 3 expression in cell or tissue lysates.

STX3 is a plasma-membrane t-SNARE that assembles into SNARE complexes driving vesicle fusion, and it is enriched at the apical surface of polarized epithelial cells, where it participates in apical trafficking and regulated exocytosis. The reagent can therefore inform studies of SNARE-mediated fusion events, secretory pathway activity, and changes in STX3 abundance linked to epithelial polarity, neurotransmitter release, and retinal synaptic function. Researchers comparing rodent and human samples benefit from the cross-reactive polyclonal response under one standardized protocol.

Because the reagent is unconjugated, the readout can be configured by pairing it with a compatible anti-rabbit IgG secondary and a chemiluminescent, colorimetric, or fluorescent detection platform. The polyclonal format provides coverage of multiple STX3 epitopes, which can help capture the protein when expression is low or when alternative splicing produces isoforms such as STX3A or STX3B. For routine handling, store at -20 °C and limit freeze-thaw cycles to preserve IgG activity over the working life of the vial.