Anti-STAT5B Antibody

This rabbit polyclonal antibody from Antibodies.com targets STAT5B, a member of the STAT family of transcription factors activated downstream of cytokine and growth factor receptors. Raised in rabbit hosts and supplied as an unconjugated preparation, the reagent is validated for Western Blot (WB), Immunohistochemistry (IHC), and Immunofluorescence (IF) workflows. The antibody recognizes STAT5B in Human, Mouse, and Rat samples, enabling comparative studies of JAK-STAT signaling in mammalian model systems and primary tissue specimens.

STAT5B operates as a latent cytoplasmic transcription factor that becomes phosphorylated at a conserved tyrosine residue following cytokine stimulation, after which it dimerizes, translocates to the nucleus, and drives expression of genes governing cell proliferation, differentiation, and survival. Researchers commonly apply this reagent to interrogate growth hormone, prolactin, and interleukin-2 family signaling, along with mammary epithelial development, lymphocyte maturation, and NK cell function. Each vial is supplied as 100 µl of liquid antibody, ready for titration in immunoblotting of total and phosphorylated STAT5B, chromogenic or fluorescent IHC on fixed sections, and indirect immunofluorescence imaging. Standard handling practice is to aliquot upon arrival and limit repeated freeze-thaw cycles of the working stock.