Anti-Stat5 Antibody

Stat5 (Signal Transducer and Activator of Transcription 5) is a key mediator of the JAK-STAT signaling cascade, and this unconjugated rabbit polyclonal reagent from Antibodies.com provides a robust detection tool. Stat5 exists as two closely related isoforms, Stat5a (~94 kDa) and Stat5b, both activated by phosphorylation at a conserved tyrosine residue following cytokine or growth factor stimulation. Once modified, the protein dimerizes, translocates to the nucleus, and drives transcription of genes controlling lymphocyte development, mammary differentiation, and hematopoietic cell survival.

This lot is validated for Western Blot, Immunohistochemistry, and ELISA applications and cross-reacts with Human, Mouse, and Rat samples, enabling investigations across cell line panels and primary tissue specimens. Researchers commonly apply it to dissect IL-2, IL-7, prolactin, and erythropoietin signaling, monitor constitutive Stat5 activation in hematological malignancies, and probe regulatory T cell biology through FOXP3 induction pathways.

The antibody is supplied as a 100 µl volume. For long-term preservation, store at -20°C in working aliquots to minimize freeze-thaw cycles; for frequent use, refrigerated storage at 4°C is acceptable for short periods. Each batch is quality-controlled by Western Blot against recombinant Stat5 to confirm specificity.