Anti-STAT3 (phospho S727) Antibody

This rabbit polyclonal IgG antibody from Antibodies.com specifically recognizes STAT3 phosphorylated at serine 727, a post-translational modification within the C-terminal transactivation domain that enhances maximal transcriptional output of the JAK-STAT signaling cascade. Raised in rabbit and supplied unconjugated at 50 µl, the reagent is validated for Western blot (WB) detection of the human phospho-STAT3 (S727) target and is widely used to monitor cytokine-driven activation in cultured cell lysates, tumor line models, and primary tissue specimens relevant to oncology and immunology research. As a polyclonal reagent, the antiserum contains a population of clones that recognize multiple epitopes surrounding the phospho-S727 site, which can strengthen the WB signal when the modified residue is partially occluded by neighboring sequence. The antibody is provided in a vial format intended for aliquoting upon arrival; standard practice is to store at -20 °C in working aliquots to limit freeze-thaw cycles and to avoid repeated exposure to ambient temperatures. Researchers investigating IL-6, interferon, or growth-factor-mediated STAT3 activation, or profiling tumor samples in which constitutive STAT3 activity has been implicated, can pair this reagent with total STAT3 controls to track phosphorylation dynamics across treatment time courses. The unconjugated format also allows the antibody to be combined with a user-selected secondary reagent for chemiluminescent or fluorescent WB workflows.